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Veterinary Diagnosis of Brucellosis and Tuberculis: a new White Paper released.

Diachemix and BioTek announce the release of an Application Note for use of BioTek Synergy 2 instrument with Brucella Fluorescence Polarization Assay test.

Contagious diseases in livestock herds represent a serious public health threat as well as causing significant economic loss. Brucellosis infection in ruminants is highly contagious and causes abortion or premature calving of recently infected animals. Mycobacterium bovis (M. bovis) is a slow growing anaerobic bacterium that is the causative agent for tuberculosis in cattle.

Both of these agents have the ability to infect a number of other animals including humans. The presence of antibodies against these pathogens in bovine serum is indicative of infection.

Fluorescence polarization is a fluorescence detection technique first described in 1926 by Perrin. It is based on the observation that fluorescent molecules, when excited by polarized light, will emit polarized light. In solution, the polarization of the emitted light is inversely proportional to the molecule’s rotational speed, which is influenced by solution viscosity, absolute temperature, molecular volume and the gas constant. If one keeps viscosity and temperature constant, then the key variable for rotational speed differences is molecular volume or molecular weight.

BioTek Application Notice - April 13, 2008